Describe Your Accomplishment Essay Example for Free

Describe Your Accomplishment Essay Describe the accomplishments you have achieved during this course. My first accomplishments are to pass the course of math and work hard on the exam. I’m accomplishment in getting a strong foundation in this course. This accomplishment is to understanding many areas covered in math, for examples: whole number, the LCM prime factorization, and simplifying the expressions, Bar graphs and line graphs, proportions, rates and unit prices and ratios, percent problems, using the equations, simple and compound interest. These are example I have achieved this school quarter. Discuss one or two topics or concepts that have been a struggle so far. The concepts that I have a struggle with this term start with rounding and estimating like for example, I would have something like maybe 47 but I know how you round to the closer which will be 50 and if the whole number is 1. The second concept I been struggle with has been simple and compound interest has been the worse. For example: If I had $1000 into and investment for 1 year. I learn the $1000 is called the principal. If the yearly interest rate is 8%, in addition to the principal, you get back 8% of the principal you get back 8% of the principal will be for the use of the money overtime. 8% of $1000 you can does like 0. 8 $1000 or $80. 00. Describe one action step you can take to overcome your struggle. One of my action step, I’m trying very hard to overcome my struggle to me would be work more on my online classes, learn to turn homework in on time and talk to my Professor more if any probably occur in my math course. Another struggle has been simple and compound interest use the practice to study before taking any type of test whether you r class or other Professor. I try to use the study plan this has help me accomplishments and make good grade. This has been a course I ready need to pass fail this same class last semester.

Isolation, Cloning, and Translation of Plasmid DNA

Isolation, Cloning, and Translation of Plasmid DNA Abstract: The objective of this experiment was to clone a kanamycin gene into the MCS of a pUC18 plasmid, and then to transform cells with the plasmids. Purified pUC18 and pKan plasmid samples were obtained. A 0.7 % agarose gel was prepared, and the wells loaded with the plasmid samples. Restriction endonucleases were used to cut a kanamycin resistance gene from a pKan plasmid. DNA ligases were used to ligate the kanamycin resistance gene on to the multiple cloning site of the pUC18 plasmid. Escherichia coli (strain DH5ÃŽÂ ±) were then transformed with plasmids. The presence of the kanamycin resistance gene in the pUC18 was determined using the indirect (pUC18 selection) and direct selection methods. The results from the gel image were inadequate. Zero colony counts were recorded on the kanamycin plates for the indirect selection method. Zero colony counts were recorded on the kanamycin/ carbenicillin plate for the direct selection method. In Conclusion it can be said that although the kana mycin gene should have been inserted into the pUC18 plasmid, the results from both selection methods indicate that it wasnt. Introduction: DNA cloning is a process in which a certain piece of DNA is replicated several times [1]. This process in essence involves isolating the gene or DNA fragment of interest, and transferring it to another molecular of DNA [1]. In order for the cloning process to begin, the DNA of interest has to be cut at precise locations [1]. Specific endonucleases are used for this process. After which a small molecule of DNA is chosen, that has restriction sites that are complementary to the DNA of interest and is capable of self-replication [1]. These small molecules of DNA are called cloning vectors (phages, plasmids, Yeast Artificial Chromosomes, or Bacterial Artificial Chromosomes can be used) [1]. The two pieces of DNAs (the vector and DNA of interest) can be joined together by using a DNA ligase [1]. The newly formed composite DNA molecule is called a recombinant DNA [1]. The recombinant DNA can then be introduced into a host cell by a process of transformation [1]. Once transformed multiple c opies of the host cell can be produced, and in doing so multiple copies of the DNA are also produced [1]. Bacterial DNA can carry genes for antibiotic resistance [2]. The antibiotic resistance gene can either be on the chromosomes or on other external chromosomal pieces of DNA (e.g. plasmids) [2]. The pUC18 is a cloning vector plasmid that contains an ampicillin resistance gene [2]. On the other hand the pKan plasmid contains a kanamycin resistance gene [2]. The pUC18 plasmids are extremely useful for transformation with an Escherichia coli host cell [2]. The pUC18 plasmid consist of an origin of DNA replication, pBR322 derived ampicillin resistance gene, and a lacZ gene of E.coli [2]. The lacZ gene is part of something called the lac operon [1]. The lac operon in essence consists of the lacZ, lacY, and lacA genes [1]. The combination of the three genes allows the cell to utilize lactose [1]. When sufficient quantity of lactose is available, the cell is able to utilize the lactose by producing the enzyme beta-galactosidase [2]. pUC18s lacZ gene contains a collection of different restrict ion enzyme recognitions sites [2]. This site within the lacZ gene is called a Multiple Cloning Site (MCS). The MCS of the pUC18 plasmids can be recognized by a number of different enzymes; hence cuts can be made at various different places [2]. In gene cloning experiments, X-gal (5-bromo-4-chloro-3-indolyl,-D-galactoside) is used to indicate the presence of the lacZ gene, and hence indicates whether or not a cell is producing the enzyme beta-galactosidase [2][3]. This indication is given by a blue coloration of the colonies growing on a medium containing X-gal [2]. Beta-galactosidase cleaves X-gal into D-galactoside and 5-bromo-4-chloro-3-indole [3]. The actual presence of 5-bromo-4-chloro-3-indole is what causes the colonies to true blue [3]. The pKan plasmid contains the kanamycin resistance gene. In this experiment the kanamycin resistance of the pKan plasmid will be cloned into the MCS of the pUC18 plasmid [2]. This new recombinant DNA will then be transformed into an E.coli strain DH5ÃŽÂ ± host cell [2]. A brief overview of the isolation, cloning and transformation processes are given above [2]. This process in the end will yield an E. coli strain that is resistance to both ampicillin and kanamycin [2]. As mentioned earlier, the multiple cloning sites (MCS) of the pUC18 plasmid is located with its lacZ gene [2]. This means that when the kanamycin resistance gene is inserted into the multiple cloning sites, the lacZ gene is disturbed [2]. This alters the production of beta-galactosidase [2]. Hence the E.coli cells are not able to utilize X-gal on a growth media, producing white colonies instead of blue [2]. The presence of white colonies can be used as an indication for insertion of the kanamycin gene in pUC18 plasm id [2]. A kanamycin/ampicillin selective media can also be used to make sure that the pUC18 plasmid has the kanamycin gene inserted into it [2]. In summary the main objectives of this experiment is to clone a kanamycin gene into the MCS of a pUC18 plasmid, and then to transforms a cell with the plasmids. The hypothesis is that a kanamycin resistance gene will be inserted onto the MCS of the pUC18 plasmid, and as a result the cells will be resistant to both antibiotics. Materials and Methods: The following materials and methods are taken from: Hausner, M., Jong, M. (2010). Experiments in Biotechnology (BLG888 ed.). Toronto: Ryerson University. Pg 7-19 Materials: Bacterial plasmids, restriction enzymes, solutions and media used: Overnight cultures of DH5ÃŽÂ ±/ pUC18 and MM294/pKan (5x10mL) were used. DNA solution kit that was used consist of solution 1 (glucose/Tris/EDTA to which lysozymes were added), solution 2 (SDS/NaOH), and solution 3 (KOAc). Enzymes RNAase (5mg/ml) and DNA ligase were used. Isopropanol and ethanol were used. TE buffer used contained 10Mm TRIS and 0.1 mM EDTA. Tris borate buffer that was used contained (TBE)(1X)10.8g Tris, 5.5g Boric acid, 10 mM EDTA, and up to 1000 ml distilled water. DNA loading dye and Ethidium bromide solution were used. The plasmids pUC18 and pKan were used. The restriction enzymes that were used were BamHI (Bacillus amyloliquefaciens H.) and HinDIII (isolated from Haemophilus influenza). 5M ammonium acetate was used. Phenol:chloroform:isoamyl was used. 50mM EDTA was used. 5 x ligation and restriction buffers were used. TE buffer that was used contained 10Mm Tris, 0.1 Mm EDTA. Cell culture of E. coli strain DH5ÃŽÂ ± was used. 50 ml of LB broth and 3 sterile saline tubes. 2 LB plates, 8 LB + carbenicillin (carb), and 3 LB + carbenicillin (carb) + kanamycin (kan) plates were used. X-gal solution was used. 1 plate of LB+ kanamycin (kan). Methods: Preparation of the plasmid DNA: pUC18 and pKan plasmid were prepared over a period of three days (three weeks). Two centrifuge tubes with the culture sample were centrifuged for 10 minutes and supernatant discarded. 100Â µl of solution 1 was added followed by 10Â µl of RNase. After 20 minutes solution 2 was added. Five minutes later ice cold solution 3 was added, which was centrifuged 10 minutes later for 10 minutes. 400Â µl of the supernatant was extracted to a clean tube, to which 400Â µl of isopropanol was then added and was left for 30 minutes at -20oC. The DNA sample was then centrifuged and the pellet speed vac. The dry pellet was re-suspended in 20Â µl of TE buffer. A gel was prepared with accordance to steps in the lab manual. The DNA samples were then loaded on to the wells and the electrophoresis apparatus ran. The gel images were taken to see presence of the pUC18 and pKan plasmids. Endonuclease restriction digestion of the plasmids and ligation of the kanamycin fragment to pUC18: Two centrifuge tubes were prepared from 10Â µl of pUC18 and 10Â µl of pKan plasmids. To each tube restriction buffers, restriction enzymes and sterile water were added (refer to the lab manual for details). The prepared tubes were centrifuged and left in a water bath. 5Â µl of EDTA was added to each tube. 100Â µl of TE buffer and Phenol:chloroform:isoamyl were added. The tubes were then pulse centrifuged and top layer remove and transferred to new tubes (A1 and B1). 100Â µl of Phenol:chloroform:isoamyl was added, top layer removed and transferred to new tubes again (A2 and B2). Ammonium acetate and ethanol were added to tubes A2 and B2. The tubes were centrifuged, supernatant discarded, pellet speed vacuumed, and finally re-suspended in TE buffer. Tube C and D were prepared with accordance to the lab manual. The new tubes were then centrifuged and incubated. Transformation of an ampicillin sensitive E.coli Strain: The first five steps to prepare the cell culture of DH5ÃŽÂ ± for transformation were done by the lab staff. Details on the steps can be found in the lab manual. Four centrifuge tubes were prepared. Tube 1 contained uncut DNA plasmids, tube 2 contained DNA sample from tube C, tube 3 contained DNA sample from tube D, and tube 4 contained sterile water. The pre-prepared cells were then added to the tubes and heat shocked. LB broth was added to each tube and incubated for 20 minutes. X gal was spread evenly on the 8 LB+ carb plates. 100Â µl from tubes 1, 2, and 4 were spread on 3 of the LB+carb+X-gal plates. 100Â µl from tube three was then plated on the remaining five LB+carb+X-gal plates. Tube 3 was also plated on to 3 LB+carb+kan plate. A dilution series (using 0.1Â µl from the previous) was prepared from tube 3 using 3 sterile saline tubes. 10 Â µl from dilution 2 and 100 Â µl from dilution 3 were spread plated onto 2 LB plates. Colonies from each plate were counted. Blue and white colonies from tube 3 plates were then streaked on to a LB+Kan plate. Results from the LB+Kan plates were then recorded. Additional details can be found in the lab manual: Hausner, M., Jong, M. (2010). Experiments in Biotechnology (BLG888 ed.). Toronto: Ryerson University Results: Figure 1: 0.7 % agarose gel digest showing the presence of the pUC18 and pKan plasmids. Lane 3 and 2 were used by Abbas and Jamie. The figure above shows the 0.7% agarose gel image showing the presence of pUC18 and pKan plasmids. If banes appeared in the respective lanes, the plasmid samples would be used in the next part of the experiment. The image above shows bands appearing for lane 3 (pKan), but none for lane 2 (pUC18). This indicates the presence of the pKan plasmid but absences of the pUC18 plasmid. Hence due to inadequate results, additional plasmid sample were prepared by the lab staff. In total results from all 14 plates were recorded. Indirect Method: Table 1: Results for colony counts for the indirect (pUC18) selection method on LB+ carb+ X-gal plates Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Tube 1 TMTC-Blue N/A Tube 2 5-Blue Tube 4 0 Tube 3 40 Blue/ 5 White 55 Blue/ 15 White 79 Blue/ 22 White 65 Blue/ 3 White 54 Blue/ 12 White The results for tube 1, 2, 3, and 4 plated on the 8 LB+ carb+ X-gal plates are shown above. Tube 1 contained an uncut plasmid which explained the high number of colonies for plate 1. Tube 2 contained a cut pUC18 plasmid, which can be explained by only 5 colonies. Tube 4 contained only sterile water; hence zero colonies appeared on the plates. Tube 3 was plated on 5 plates, showing an average of 59 blue colonies and 11 white colonies. Direct Method: No colonies were obtained from the three plates of LB + carb + kan plates. Competent Cell and Percentage Transformation Calculation: The dilution series was prepared from tube 3, as indicated in the materials and methods section. Dilution 2 had a 100 colonies and dilution 3 had 30 colonies. The CFU (colony forming unit) calculations and values are shown below. CFU = (# of colonies) x (dilution factor) / (volume plated) CFU for dilution 2 = 100 x 104/ 0.1 = 10000000 cells/ml CFU for dilution 3 cant be calculated because it doesnt fall between the 30-300 colony limit. Table 2: Percentage transformation of colonies using competent cells (CFU) Plate 1 Plate 2 Plate 3 Plate 4 Plate 5 Average Percentage Transformation For total colonies (%) (45) 0.0045 (70) 0.007 (101) 0.0101 (68) 0.0068 (66) 0.0066 (70) 0.0070 In order to calculate percentage transformation, calculations from CFU are need. Percentage transformation can be calculated using the total colonies (i.e.. plate 1: 40 blue+5 white =45 total). Percentage Transformation = (Transformed cells per ml /competent cells (CFU) )x 100% So for example for the average of 70 total colonies; =0.007% Discussion: As mentioned in the result section the agarose gel image was inadequate. Lane 2 and 3 in figure 1 represent the pUC18 and pKan plasmids respectively. Clear bands were seen for the pKan plasmid however this is not the case for the pUC18 plasmid. In order for the plasmids to show up, they had to be extracted from their respective E. coli strains(pUC18 (DH5ÃŽÂ ±) and pKan (MM294). The presence of bands on the pKan lane proves that there is actual extraction from the cells. The presence of multiple bands could indicate the presence of multiple size plasmids of pKan. The fact that no bands were seen for pUC18 could be as a consequence of inadequate extraction from the E. coli cells (DH5ÃŽÂ ±). Experimental procedural error could have resulted in this. Both strains of microbes would have been genetically engineered to only contain the plasmid of interest; hence the risk of contamination is reduced. The selection methods for the experiments were divided into indirect (pUC18 selection) and direct selection methods. As mentioned in the materials and method section, cells from tube 1 were streaked on to a plate. The cells were transformed with undigested pUC18 plasmids. The colonies were too many to count and were all blue. The high number of colonies could simply occur because of the stable natural of the undigested pUC18 plasmid. The undigested pUC18 plasmids contain an uninterrupted lacZ gene, capable of producing beta-galactosidase. Beta-galactosidase is hence able to utilize X-gal on the plates and produce the large number of blue colonies. Since the cells were carbenicillin resistance (due to the pUC18 plasmids), they were able to grow on the plates. Cells were transformed with digested pUC18 plasmids from tube 2. Cells from tube 2 formed too few colonies (only 5) when compared to tube 1 (TMTC). This is due to the unstable nature of the digested pUC18 plasmids. These plasmids were digested with HinDIII and BamHI, and it possible that not all of them had an opportunity to re-ligate properly. The restriction enzymes could have cut up the lacZ gene or the carbenicillin (ampicillin) gene making it difficult for the plasmid to come back to its original conformation and survive on the X-gal+carb plate. A large majority of the pUC18 could have been cut in to smaller fragments rendering then inactive. Tube 3 initially contained the digested pUC18 and pKan plasmids. Cells were then transformed with the content of this tube. Since the transformation process is not perfect, there is no way to know what plasmid the cell took up. Hence it can be assumed that cells were transformed with either only the pUC18 plasmids, the pUC18 plasmids with the kanamycin gene, pKan and Puc18 or in some case only the pKan plasmid. Five plates were spread plated with these cells and presence of blue and white colonies were noted. As the results indicate a mixture of both blue and white colonies were obtained with an average of about 59 blue colonies and 11 white colonies. Blue colonies would hypothe tically contain cells (plasmids) with an intact lacZ (producing beta-galactosidase) gene justifying the blue color. The white colonies would have there lacZ gene disturbed (not producing beta-galactosidase), because another piece of DNA would have been inserted into the MCS. However the production of white colonies doesnt dictate the insertion of the kanamycin gene into the pUC18 plasmids. It is highly possible that another gene or DNA fragments from the pKan plasmid got inserted in the pUC18 plasmids. Confirmation of this was performed by streaking white colonies onto a kanamycin plate. The fact that no colonies grew, indicated that the kanamycin gene was in fact not inserted. This proves that the results are false positive because white colonies appeared on the X gal plates, but didnt on the kanamycin plates. This means that the white colonies werent transformed with what we wanted. Finally it is noted that when tube 4 was streaked on to a plate, no growth occurred. This seems log ical as the cells in this tube were only transformed with sterile water, which means no plasmids were present. The cells would not have contained plasmids with the carbenicillin resistance gene, and hence did not survive on the carbenicillin plates. The direct method results were recorded from the LB + carb + kan plates. No growth was observed in any of the plate, which proved to be highly contradictory to our hypothesis. Presence of white colonies on the indirect method plates but none on the direct method plates was suprising. White colonies were assumed to have pUC18 plasmids with both kanamycin and carbenicillin resistance genes. Hence its inability to grow on the carb + kan plates was surprising because white colonies grew on the X-gal plates. However as mentioned earlier it could be possible that another fragment of DNA was inserted into MCS besides the kanamycin gene. The fact that white colonies also didnt appear when they were streaked on to a kanamycin plate, ties in with these results. Both direct and indirect methods have their own advantages and disadvantages. Indirect method involves multiple steps and hence in many cases can be time consuming. More plates are involved in the indirect methods, making it difficult to keep track sometimes, also adding to cost. However the indirect method helps to indentify the false positive/false negative results. The indirect selection method helps to make a comparison between the cut and uncut pUC18 plasmids. Comparison of the colonies shows the effect of restriction enzymes of the activity of the pUC18 plasmids. Moreover the indirect method is much more selective. This is because it first shows which colonies have an insertion in the multiple cloning site through the blue/white screening method. Then the plating of these white colonies on to a kanamycin plate helps to confirm that it was a kanamycin resistance gene that was actually inserted (on the MCS). The direct method is very concise involving only one plate, which save b oth time and money. This selection method has no chance of giving false negative/false positive results. The direct selection method selects for cells that have been transformed with pUC18 plasmids, and have a kanamycin resistance gene in their MCS. Since the pUC18 plasmid already has an ampicillin resistance gene (carbenicillin in this case), the insertion of kanamycin resistance gene allows it to survive on a LB+carb+kan plate. A problem comes when the plasmids dont have the necessary gene inserted in their MCS. So in this case for example it could be possible that the plasmid doesnt contain the kanamycin gene so the kanamycin antibody kills it, even though the carbenicillin resistance gene is there. Another technicality comes when a cell transformed contains both pUC18 and pKan at the same time. Because this selection method only selects for cells that have both carbenicillin and kanamycin resistance, it is difficult to tell whether the cell selected has both plasmids (pUC18 and pKan) or only a pUC18 (with the kanamycin gene). Therefore although more time consuming the indirect method is more useful. Some of the experimental errors that occurred could have been due to improper spreading techniques. The process of cell transformation that was used was through heat shock. It could be possible to use other cell transformation technique such as electroporation. In Conclusion it can be said that although the kanamycin gene should have been inserted into the pUC18 plasmid, the results indicate that it wasnt.

The Pleasure Image Of Honolulu Tourism Essay

The Pleasure Image Of Honolulu Tourism Essay When we say Hawaii, the first things that come to our mind are nice weather, sandy beaches and relaxation. For my assignment I chose the capital and largest city of Hawaii, Honolulu. The reason of choosing this heaven on earth as the convention destination is not only because of its personality as the city of pleasure, but also because Honolulu represents an economic centre and principal port of the Hawaiian Islands where business can be conducted. Due to the fact that Hawaii ranks among the top 3 destinations for leisure travel in the US, Honolulu is a location that attracts a possible event clientele. From the pleasure side, Honolulu has an agreeable year-round climate, so planned events could be held at any given time of the year. Due to the fact that Honolulu has the lowest rate of violent crime of any US city, visitors can feel safe and secure. Besides enjoying the great climate and swimming on the magnificent beaches, the spectacular views of active volcanoes on the outlying islands also represent an attraction. The wealth of natural beauty combined with the splendid volcanoes offers a truly great choice for tourists. However, regardless of all the beauty of the volcanoes, tourists primarily are coming because of sun, sea and beaches. Several beaches are covered with black and green sand, which leaves no one indifferent. Their banks are covered with finest black and green stones, which are incurred by volcanic activity. The color of the beach combined with the beautiful blue oceans is unforgettable. Honolulu is a popular tourist destination and Surfers Paradise. The most famous and popular local beach is Waikiki Beach. Waikiki Beach has numerous hotels. Above the town rises the volcanic crater Diamond Head. From the top of the volcano is a beautiful view of Honolulu and surrounding areas. A further tourist attraction is the famous military base of Pearl Harbor. Additionally, in the surrounding areas are bamboo forests. Additional places of interest are: Aloha Tower, is described as the beautiful 10-story Aloha Tower, one of Hawaiis best-known landmarks. It is built in 1926 when all travels were done by boat. The tower was created in order to make an impression for tourists during entering and leaving the harbour. Kapiolani is Honolulus largest park, which contains a zoo, an aquarium, and the well -known Waikiki Shell structure. The Honolulu Botanical Gardens which contains four gardens in and around the city. The Arizona Memorial, for the 1,100 who died during the bombing of Pearl Harbor. The University of Hawaii The Bishop Museum, well-known for its studies of Polynesia. The Honolulu Academy of Arts, known for its Asian and Hawaiian collections. Kawaiahao Church (1841), where funerals for Hawaiian monarchs and nobility were held. Iolani Palace, the previous home of Hawaiis kings, which represents the only royal palace in the United States. The factors that affected the citys growth as the business centre of Hawaii are: geo-political position of Honolulu; tourism development; diversification of industry; development of harbour facilities; the achievement of an international airport; constructions of luxury hotels; natural habitats of endemic species; ores; forests and volcanoes. Because of all this, Honolulu attracts a large number of tourists yearly. The Hawaiians make several hundred billion dollars annually selling sea, sun, palm trees and its superior climate. THE BUSINESS IMAGE OF HONOLULU From the business point of view, Honolulu has the remarkable 1.1 million-square-foot Hawaiian Convention Centre. Moreover my research indicates that Honolulu has hosted conferences and events for many well-known corporations, including: The National Psychological Association National Medical Association Hawaiian State Department of Health As a result of developed tourism, the population is focused on tourism and hospitality. During the past 100 years and due to its many advantages, the island attracted investors from the United States to invest considerable sums of money in its development. Honolulus current industry foundations include tourism, followed by: federal defence expenditures; agricultural exports (chiefly pineapples); telecommunications and mining. Moreover, worthwhile mentioning industries of Honolulu are: jewellery; printing and publishing; clothing; food and beverages; rubber products; construction materials and electronics and computer equipment. Additionally, Honolulu represents the regional headquarters for many well-known companies such as: Hawaiian Airlines; Bank of Hawaii; Oahu Transit Services, Inc. and Kaiser Permanente Medical Group. In addition, this city has several colleges and universities, including the University of Hawaii-Manoa, Hawaii Pacific University and Chaminade University of Honolulu. MEETING SERVICE PROVIDERS Based on my research extra meeting service providers in Honolulu are: The Catering Connection Unlimited Inc. represents the Award Winning Catering Connection Unlimited. It offers detailed execution, superior quality, creative culinary creations and complete beverage services. Creations in Catering represent Food Services famous for creating and producing successful on-premise and off-premise catered functions and special events. Creations in Catering is Known in the industry for producing award-winning presentations, Creations in Catering has gained recognition on the international, national and local levels for its expertise and creativity. Production Hawaii is Event Rental Dà ©cor service which offers event equipment such as Clear-span structures, Canopy tents, Stages, Portable floors, Carpeting, Dance floors, Tables, Chairs, Bleachers, Air conditioners, and Pipe Drape Trade Show Booths. Anthony Calleja Photography is Production Design Service by a professional photographer and artist whose specialities include Commercial, Corporate, Events, Banquet Dinners, Conventions, Product, and Stage Performances. CONVENTION AND VISITOR BUREAUS SERVICES Convention and visitor bureaus are organizations which offer to the meeting planners access to a variety of services, packages and value-added extras through meeting preparation, planning and applying. The main functions of CVBs are locating meeting places, checking hotel availabilities, arranging events. The advantages that CVB facilities offer to meeting planers are: Access to a range of services and value-added extras through a bureau. Help for developing the convention schedule through the creation of pre and post-conference activities, spouse tours, and hosting of special events. Direction for products and services that will work best to accommodate clients needs and budgets. Mediatising and matching meeting needs to the products, services, and speakers available in a community. Connecting planners with the suppliers, from motorcoach companies and caterers to off-site entertainment venues. Offering information about services and facilities in the destination. Informing about local events with which your meeting may beneficially coincide (like festivals or sporting events). Providing hotel room count and meeting space statistics, keeping a convention/meetings/events calendar in order to help planners avoid conflicts and/or space shortages. Matching properties to specific meeting requirements and budgets. Work with city government to get special permits and to cut through formalities. Some of the specific services that can be offered to the meeting planners are as well: collateral material ; help with on-site logistics, including registration ; housing bureaus ; supplementary services, for example production companies, catering, transportation ; speakers and local educational opportunities ; security ; coordination of local transportation and access to special venues. Hawaii Visitors And Convention Bureau http://custom.cvent.com/33CBACD109164CAB81B5F1D8FEA72786/pix/RFP/CF55FB69F5CF4D158028D9B5B66451AA/8e9114ca3d454f94aad464c7693ef81b.jpg 2270 Kalakaua Avenue Suite 801 Honolulu HI 96815 Listing/VenueOverview.aspx?ofrgstub=cf55fb69-f5cf-4d15-8028-d9b5b66451aapnum=1so=1returl=%2fRFP%2fVenues.aspx%3fma%3d47%26vtt%3d1%26wt.mc_id%3dDG_Right_Nav%26vt%3d32ckm=L1JGUC9WZW51ZXMuYXNweD9tYT00NyZ2dHQ9MSZ3dC5tY19pZD1ER19SaWdodF9OYXYmdnQ9MzI= 21.275800 -157.823400 3 CVB According to the Hawaiian Visitors and Convention Bureau description, Hawaii is world renowned destination of breathtaking natural beauty. Despite the image of a dream destination, Honolulu is a centre of international commerce and business meetings. AVAILABLE HOTELS AND CONFERENCE CENTRES FOR SPECIAL EVENTS My research points towards available hotels and conference centres as follows: The Hawaiian Imin International Conference Centre is designed for an international audience, offering outstanding resources to produce successful events of all kinds. The Hawaiian Convention Centre is easily located closest to the hotel-plentiful Waikiki Beach, which offers more than 30,000 hotel rooms. The centre is located 7.5 miles of The Honolulu International Airport .The Hawaii Convention Centre gives to the guests opportunity to take pleasure in Hawaii from inside. As it described more than 60 percent of the centre is landscaped with palm trees and green tropical plants. The Centre includes tropical garden and waterfall. Worthwhile mentioning is the fact that the centre was voted the most beautiful convention centre in the world by the International Association of Exhibition Management and is the winner of nine consecutive Prime Site Awards. The convention center has nearly 150,000 square feet of meeting space, cutting-edge technology and delicious Hawaiian cuisine. Hyatt Regency Waikiki Beach Resort and Spa according to the description represents an ideal place for planning an event by reason of premier meeting rooms set in the heart of a tropical island getaway. The hotel as it is described features 19,500 square feet of function space, 2,050 square feet of pre function space, and 9,800 square feet of exhibit space. Moana Surfrider, A Westin Resort Waikiki Beach is located in the heart of Waikiki, with short walking distance from shopping, dining, and attractions. It includes more than 12,000 square feet of gathering space provides the perfect setting for parties, weddings, or business functions. As it described, there are eight meeting rooms ranging from the intimate 780-square-foot Board Room to our 4,340-square-foot Ballroom, which can host a reception for up to 300 guests. Outdoor spaces include the simply outstanding Diamond Head Lawn Terrace with breathtaking views of Diamond Head and the Pacific Ocean. The beautiful Roof Garden and Roof Garden Lanais are ideal for small to mid-size meetings and receptions for up to 50, and our private beach perfect for a sunset wedding for up to 150 guests. Best Western The Plaza Hotel is located just half mile from Honolulu International Airport and Honolulu Military Bases.The hotel is nestled between downtown Honolulu, Waikiki and the military bases, close to some of Honolulus major business districts. The hotel has four different size banquet facilities, enabling to accommodate from 10 to 200 guests. Hawaii Prince Hotel Waikiki Hawaii offers excellent facilities and services as well as the perfect location for events. The Hilton Waikiki Prince Kuhio hotel is located in the heart of Waikiki, closed to the famous Waikiki Beach. Apart the good location, the hotel has flexible meeting facilities of 17,000 sq ft. The Doubletree Alana Hotel Waikiki is located at the entrance to Waikiki in Hawaii. As it described, hotel is four miles from downtown Honolulu, and short walk from Hawaii Convention Centre. Turtle Bay Resort represents a breathtaking background for meetings and events. Featuring 31,000 square feet of function and pre-function space it represents an ideal destination for events. THE OBSTACLES OF HONOLULU The biggest problem for the local population is constant volcanic activity, which often forced population to change residence and move the entire settlement away from active volcanoes and earthquakes, which are the results of their activities. Furthermore, a double-edged sword is the major waves that sometimes reach the height of 25 ft.   Waves are known to have destroyed the coast, particularly these associated with frequent hurricanes and tropical storms. One of the disadvantages of Honolulu is the high cost of living. Moreover, according to information given by tourists experiences, Honolulu has more cars than the Roads and Highways which can cause massive traffic. In my opinion, some of the obstacles, such as the high cost of living and the issues with the considerable traffic need to be addressed and solved. From my point of view, the costs of living should be decreased, as well as the number of cars. Furthermore, by decreasing the number of cars and developing a more organized traffic system, road congestions may be avoided. CONCLUSION: Conclusively, Honolulu interested me as a location for pleasure tourism and as an economic centre as well; what I gained from my research was that I went deeper into the analysis of the city and its facilities available for conventions and meetings. Additionally, based on my research and looking at Honolulu at the point of view of meeting planner, I learnt a lot about city facts and facilities available for organizing conventions and meeting, moreover Meeting service provides, available hotels and conference centre for special events and convention and visitor bureaus services. Feb. 6th, 2010

Cold War Essay -- essays research papers

What was the Cold War and what events caused it? Cold War is the term used to describe the intense rivalry that developed after World War II between groups of Communist and non-Communist nations. On one side were the Union of Soviet Socialist Republics (U.S.S.R.) and its communist allies that referred to as the Eastern bloc. On the other side were the United Staes and its democratic allies, usually referred to as the Western bloc. Cold War was characterized by mutual distrust, suspicion, and misunderstandings by both the United States and the Soviet Union, and their allies. The United States accused the Soviet Union of seeking to expand Communism throughout the world. The Soviets charged the United States with practicing imperialism and with attempting to stop revolutionary activity in other countries. Each bloc’s vision of the world also contributed to East-West tension. The United States wanted a world of independent nations based on democratic principles. The Soviet Union attempted to control areas it considered vital to its national interest, including much of Eastern Europe.   Ã‚  Ã‚  Ã‚  Ã‚  The Yalta Conference is often cited as the beginning of the Cold War. During the seven days of February 4 – 11, 1945, the Big Three – Franklin Roosevelt, Winston Churchill, and Josef Stalin – met in Crimea at the Lavidia Palace on the Black Sea. The main purpose of Yalta was the re-establishement of the nationas conquered and destroyed Germany. Poland was given back it...

Perfectionism and Athlete Burnout in Elite Sports: The Mediating Role o

Over the past few decades, American society has become more and more obsessed on performance outcomes and winning; being declared the best has become most important (Crain, 2004). Winning is often viewed as an all or nothing virtue, whereby greatness is a descriptive term reserved only for those whose names appear at the top of the list (Hanchon, 2011). This evolving mindset communicates to our youth that despite his or her efforts, only the final results matter. For many individuals the ideas of achievement, excellence, and self-worth have become highly dependent upon the perceived outcomes of the competitions or events in which they engage (Hanchon, 2011). Outperforming one’s competitors serves as the defining characteristic of success or excellence, which in turn, appears to serve as a key determinant in the individual’s self-assessment of life satisfaction (Harackiewicz, Barron, & Elliot, 1998). Sport performance is mediated by positive and negative variables; the pressure to perform for a result leads to the negative variable of higher expectations on the athlete. Stress and the pressure to perform are both contributing factors to higher anxiety levels, overtraining, and burnout in athletes (Weinberg & Gould, 2007). In some cases, â€Å"higher expectations also appear to increase the amount of stress an athlete may experience, and higher levels of stress are generally related to higher levels of state anxiety and burnout† (Jones & Hanton, 1996; Raedeke & Smith, 2001). Burnout Fear of failures, frustration, high expectations, anxiety, and other pressures to perform are all stresses identified as being related to burnout (Dale & Weinberg, 1990). Burnout has been addressed in the Old Testament (Exodus 18:17-18), in which ... ...ation and affect on elite athlete burnout susceptibility. Journal of Sport and Exercise Psychology, 28, 32-48. Lonsdale, C., Hodge, K., Rose, E. (2009). Athlete burnout in elite sport: A self-determination perspective. Journal of Sports Sciences, 27, 785-795. Raedeke, T. D. (1997). Is athlete burnout more than stress? A commitment perspective. Journal of Sport and Exercise Psychology, 19, 396-417. Raedeke, T. D., & Smith, A. L. (2001). Development and preliminary validation of an athlete burnout measure. Journal of Sport & Exercise Psychology, 23, 281-306. Vallerand, R. J. (2008). On the psychology of passion: in search of what makes people’s lives most worth living. Canadian Psychology, 49, 1-13. Weinberg, R. S., & Gould, D, (2007). Foundation of sport and exercise psychology (4th ed.). Chapter21: Burnout and Overtraining (pp. 489-509). Champaign, IL: Human

A Journey into the Heart of American Adolescence

Teenagers will be teenagers.   Perhaps this is the best way to understand the lives of eight teenagers in Hersch’s (1999) book, A Tribe Apart: A Journey into the Heart of American Adolescence.   Although Hersch only writes about American teenagers, adolescents around the world may be able to relate to the eight kids interviewed by the author.They are naughty, to say the least, and their parents seem to have little or no interest in how they are leading or in fact ruining their lives.   The teenagers use illegal drugs, enjoy premarital sex, steal, get into trouble, and essentially do everything that they are most likely to do in the absence of adults from their lives.Adults have abused them through neglect or other means.   Hence, the young people do not have real models to follow.   Instead, they experiment with life so as to learn their own lessons before adulthood strikes.   Many of the lessons that such teenagers may learn will undoubtedly be painful if not plai n sad.It is clear to the reader of A Tribe Apart that these teenagers could have been saved from the difficulties they may inevitably face by following models of propriety.All the same, it is impossible to find such models when their parents are missing from home and out at work.   Teachers may not be able to fill in the gap seeing as it is the parents’ responsibility to teach morality to their kids for the latter to consider it believable.   After all, children are meant to spend more time with their parents than with their teachers.The teenagers of A Tribe Apart do not belong to poor families.   Researchers have often described adolescents from poor families who are neglected or abused by other means before they turn into drug addicts or thieves.Teenagers belonging to poor families are therefore believed by the masses to be morally degraded.   The unique fact about Hersch’s book is that all of the teenagers she has interviewed for her research belong to the h ealthy middle class.   Perhaps this makes it easier for adolescents around the globe to relate to the eight teenagers in her book.Most if not all teenagers may be considered ‘a tribe apart’ as the reader contemplates the fact that both the haves and the have-nots behave in similar ways through adolescence.   Indeed, teenagers belonging to poor families appear to be destroying their lives just like the adolescents interviewed by Hersch for her study.The good news is, however, that Hersch’s book could serve as a warning signal for parents who have neglected or abused their growing kids in other ways.   If parents do not take heed, their growing kids may very well shape themselves as adults that behave like their own parents.   Wealth does not matter in this case.   Rather, teenagers would remain as stereotypical teenagers – experimenting with adulthood in their youth.   They know no boundaries.They are always crossing their limits.   Most impor tantly, there is nobody to guide them out of their troubled existence.   Drugs and sex become the sole source of joy for them.   Thus, Hersch’s book is a wake up call that all parents must give serious thought to.   The fact that eight teenagers confided in Hersch must also be taken seriously.   It is possible for parents to honestly understand their kids.   Hersch has proved this with her research.

Environmental Assessment of the Asopos River Basin

Presentation of Asopos River BasinEtymology – MythologyAsopos: ( Grecian: I ) from Asis, Greek: I†  ( = I ) , intending â€Å"marsh ( or Moor ) † and Opsis Greek: I , intending â€Å"appearance† . ASOPOS ( or Asopus ) was a River-God of Boiotia in cardinal Greece, and Sikyonia in the Peloponnesos, southern Greece. Naiades, Asopos’ 20 girls, were H2O nymphs who had names of Greek island towns.LocationIn a study of Ministry of Environment, Physical Planning and Public Works ( MoEPPW ) in 2006, it is referred that a entire country of 12,341 kilometers2is occupied by Water District 07 of East Sterea Ellada. This country consists of the Prefecture of Evoia, major parts of the Prefectures of Fthiotida ( 83.1 % ) , Voiotia ( 98.5 % ) , Fokida ( 41.9 % ) and smaller parts of the Prefectures of Magnisia ( 14.9 % ) and Attica ( 7.2 % ) . River Basin of Voiotikos Kifissos River, River Basin of Sperchios River and River Basin of Asopos River are the chief River Basins of the Water District referred above. Other important H2O organic structures located in that country are lakes Iliki and Paralimni. In the Figure below is presented the Water District 07 of East Sterea Ellada. The entire surface country of Asopos River Basin – which is located in East Attica and Voiotia Districts ( Central Greece ) and flows from West to east – is 450 kilometer2and extends to Evoikos Gulf. The entire length of Asopos is 57 kilometer, holding its beginnings in Elikona mountain, and some watercourses from Parnitha and Dervenochoria Mountains. Its flow watercourse base on ballss through Asopia, Inofyta, Schimatari, enters the part of north-east Attica and eventually meets the sea near Oropos laguna, in north Evoikos Gulf, as shown in the Figure below. Vertical tectonic motions, of different grades of strength resulted in the creative activity of Asopos’ River Basin. That is the ground why the basin is non homogeneously developed and has differences in deposit in different places. The part studied – piece of the Sub-Pelagonian zone – has a particular geological formation. More specifically it is constituted by three chief units:the crystalline cellar stone ( schists, schists with psammitic stones, schists with marbles and sipoline embolisms )the alpine cellar stones ( limestones and dolomites of Triassic and Jurassic age )the post-alpine deposits ( Neogene lignite-bearing sedimentations, marly formations with lignite embolisms, pudding stones, marly limestones and travertines, and other coarse unconsolidated stuff ) .A hydrogeological analysis of Asopos’ River Basin, concluded that semi-pervious formations of Neogene-Quarternary, extremely pervious formations of calcite and other imperviable formations cover the 55 % , 41 % and 4 % of the River Basin severally. The unequal spring H2O flow every bit good as the extended being of formations made by karst convert precipitation to direct infiltration further restricting surface H2O flow. As a consequence, the bulk of the H2O flow derive from natural or semi-processed industrial or domestic wastewaters. In dry periods, the H2O flow eliminates highly and the sea H2O enters the estuary of the river for 100s of metres. In a research of the Institute of Geology and Mineral Exploration ( I.G.M.E. ) in 1996 the coefficients of the surface drainage and infiltration were estimated to 0.19 and 0.25 severally. In the same research the appraisal of the one-year discharge is 70.1 hectometer3.Surface and Groundwater{ The fact that the H2O of the downpours penetrates into the constructions of groundwater, due to the widespread parts of limestone made by karst, consequences in an disconnected interaction between the two sides of the channel. As a consequence the hydrographic web of Asopos is non peculiarly good developed. In the yesteryear, despite its big catchment country, Asopos itself held H2O merely for a really short period. The ground was the fast incursion of the H2O from the surface into the aquifer. Nowadays, there are sections of Asopos that have H2O event in the summer, as a consequence of smaller subdivisions that enter the river. The Asopos’ River Basin has merely specific countries with impenetrable formations ( clay sedimentations, schists ) . As a consequence the H2O flow of the watercourse is non uninterrupted, apart from little downpours that keep a H2O flow for a specific clip period ( i.e. the Lantikos and thes Gouras ) . Streams like the Liveas ( in the Northwest of Malakasa ) have seasonal H2O flow. Other streams – the longest 1s – are the Potisiona, the Sklirorrema and the Vathi ( drain in its north side ) . Streams that drain of the south side of the basin are the Lykorrema, the Xerias, the Bresiko etc. A study conducted by Ministry of Environment, Physical Planning and Public Works ( MoEPPW ) in 2006, reports that there is an indicant of pollution caused by high organic tonss caused by industrial and urban wastes every bit good as agricultural tally offs in Asopos’ catchment country. The consequences – findings of that research were high concentrations of nitrates and P in Asopos River. Equally far as the groundwater quality is concerned, its features were classified into two separate classs: the ions and the hint elements. Some of the consequences of the survey of the Institute of Geology and Mineral Exploration ( I.G.M.E. ) ( Gianoulopoulos, 2008 ) are:the chief beginning of the nitrates are the N fertilisers, which are used in the agricultural sector. Additionally, the being of ammoniacal and nitrite ions is due to the urban and industrial pollution beginnings.There is increased concentration of Cl–and PO4ions, which are consequence of industrial pollution beginnings.The figure below shows the precipitation and the average temperature of the part of Asopos for the period October 1999 to September 2010. The informations are taken from the meteoric Stationss at Kallithea, Tanagra and Marathon. As we can easy detect the one-year mean precipitation degrees are 534.5 millimeters, 502.9 millimeter and 625 millimeter severally. Equally far as the mean one-year ai r temperature is 16.7OC and 17.5OC at the Tanagra and Marathon Stationss severally.Areas of Asopos categorized by usageThe country of Asopos has H2O demands for industry, agribusiness, abode and touristry. The survey about Asopos River Basin has to take into consideration that H2O demands and measure the distribution of that needs among the utilizations. There is a demand to specify the sectors that put more force per unit area in H2O usage. For the industrial sector, H2O usage takes topographic point for rinsing and colourising ( fabrics ) , steel production, cement production, oil processing, energy production etc. Sing the touristry sector of economic system and domestic sector ( families ) H2O usage concerns the H2O supply for place usage by the authorized provider. The prioritization of H2O usage in Asopos River Basin is as follows:Water supply of families, touristic units and vacation placeIrrigation of cultivated countries and farm animal unitsIndustrial H2O usageIn the figur e below the land usage of Asopos River Basin is presented. More specifically, the ecru colour represents the agricultural usage and the light purple the industrial usage.Demographic DataPermanent PopulationThe lasting population of Asopos River Basin as reported at 2001 Census is given in the tabular array below: For the Municipalities / Communes that do non fall entirely within Asopos River Basin, merely the lasting population of the subsequent Municipal Departments and urban vicinities that are included in Asopos River Basin was calculated.EmploymentTaking a expression at the Table below, which is harmonizing to informations from the Labor Force Survey of the National Statistical Service of Greece, we observe that the entire population of working age is divided into two big classs – the economically active population and the economically inactive population. The economically active population is divided into the employed and the unemployed. The employed are people with age greater than or equal to 10 old ages, who had worked even for merely an hr during the mention hebdomad ( for wage or net income or in household concern ) . Unemployed are people with age greater than or equal to 10 old ages, who did non hold work during the mention hebdomad, were presently available for work and we re either actively seeking work in the past four hebdomads or had already found a occupation to get down within the following three months. In economically inactive population belong those individuals who neither classified as employed nor as unemployed. Taking into consideration the information given by the Table above in combination with the definitions given, we can easy detect that the entire economically active population – in other words the work force – of Asopos River Basin in 2001 was 31,764 people. Out of these people 28,837 are employed – 90.8 % of the entire economically active population – and 2,927 are unemployed – 9.2 % of the entire economically active population. Equally far as the employing sectors is concerned, in the primary sector are employed about 19.8 % of the sum of employed people and about 9.8 % of the entire population ( economically active and economically inactive people ) . In the secondary sector are employed about 28 % of the sum of employed people and about 14 % of the entire population of Asopos River Basin. Finally, in the third sector are employed about 35.2 % of the sum of employed people, whereas about 17.4 % of the entire population of Asopos River Basin. As shown in the Table below people that work in the primary and secondary sector are about 24 % ( 9.8 % + 14 % ) of the resident population of Asopos River Basin over 15 old ages old, whereas in the third sector is occupied about the 18 % of the resident population of Asopos River Basin over 15 old ages old. On the other manus in Athens country occupants over 15 old ages old who are occupied in the primary and secondary sector are 11 % , whereas those who are occupied in the third sector are about the 32 % . Overall we can detect that the primary and secondary sector are more developed in Asopos River Basin than in Athens country, whereas the third sector is much more developed in Athens country.The ProblemDescriptionTaking into history the fact that Asopos part supports 1300 industries and related installations – nutrient and drink industries, agrochemical, metal processing etc. – it is considered as the largest industrial part of Greece. 1970 was the decate that indus trial activity started in the country of Asopos and more specifically in Schimatari and Inofita. To show some Numberss for the significance of the job of this country, we refer that 130 units of the bing 1s produce waste Waterss during their operational maps ( production ) . Equally far as the waste Waterss are concerned, the entire day-to-day produced measure is about 9,044 m3/day. 84 % of that measure is due to industrial waste Waterss. More specifically, this measure is split in 7,605 m3/day and 1,439 m3/day, the entire day-to-day measure of waste Waterss of the industrial units of the country and the entire day-to-day measure of waste Waterss of the employees of these units severally. Taking a glimpse at the Table below, which is a study from a study of M. Loizidou in 2009, we can easy detect that the chief sectors from which the bulk of waste Waterss come from are the sectors of â€Å"Textile and leather industries† , â€Å"Metallurgy related industries† and †Å"Industries of Foods and Drinks† at 25 % , 21 % and 30 % severally.Consequences – ImpactsThe estimated impacts of industrial sector on Asopos River Basin are fundamentally environmental and societal impacts. More specifically the environmental impacts of industrial pollution in Asopos catchment consist in catastrophe of biodiversity – fish and invertebrates, birds on estuary – and in the pollution and decrease of groundwater. On the other manus the societal impacts of industrial pollution in Asopos catchment are impacts on human wellness – from ingestion of contaminated agricultural merchandises and groundwater, impacts on local economic system, which is because of increased cost for imbibing H2O for families, increased cost for local agricultural manufacturers, increased cost for nutrient industries, and lessening of tourers for local tourer companies. Finally, other societal impacts of industrial pollution of Asopos River Basin are impacts on diversion of local occupants every bit good as visitants ( touristry ) .Choice Experiment Method on Asopos CaseUsing the Choice Experiment Method on the Asopos Case, the research workers targeted to measure a package of betterments, which could take topographic point in the Asopos River Basin. This package of betterments includes:Environmental conditions described in footings of ecological position in all H2O organic structures of the catchmentImpact on the local economic system in footings of tourism/recreation, demand for local production and cost of life for families andImpact on human wellness described as handiness of H2O with a quality and measure sufficient for fulfilling different local utilizations.The package of betterments is a mixture of usage and non-use values. As usage value is defined the values that people derive from the direct usage of a good. Examples of usage value are runing, fishing, or boosting. Use values may besides include indirect utilizations. For illustration, a particular part offers direct usage values to the people who visit the country. Others could hold fun watching about this part in a Television show. In that manner they would have indirect usage values. As non-use value is defined the value that people assign to economic goods ( including public goods ) even if they ne'er have and ne'er will utilize it. Non-use value as a class may include:â€Å" option value † – the value placed on single willingness to pay for keeping an plus or resource even if there is small or no likeliness of the person really of all time utilizing it, happening because of uncertainness about future supply ( the continued being of the plus ) and possible hereafter demand ( the possibility that it may someday be used ) .â€Å" bequest value † – values placed on single willingness to pay for keeping or continuing an plus or resource that has no usage now, so that it is available for future coevalss.â€Å" Existence value â€Å" – an unusual and slightly controversial category of economic value, reflecting the benefit people receive from cognizing that a peculiar environmental resource, such as Antarctica, the Grand Canyon, en dangered species, Sharri Dogs or any other being or thing exists.â€Å" selfless value † – the value placed on single willingness to pay for keeping an plus or resource that is non used by the person, so that others may do usage of it. Its value arises from others ‘ usage of the plus or resource.The method of Choice Experiments was chosen because since it is a conjectural survey-based method, it can quantify the public-service corporation every bit good as the Willingness To Pay ( WTP ) for different conjectural degrees of each property examined. In add-on, in Choice Experiments the respondent chooses between options, as packages of properties, doing picks ensuing to a lower danger for strategic prejudice – yeah stating, in the replies. Finally, it is one of the best ways to measure non-market resources, options and properties.Sampling – Survey MethodExcept for the necessitate of rating of the socio-economic and environmental effects related with th e debasement of the basin, the survey that took topographic point aimed to look into the manner the two different populations – that of the occupants of Asopos ( rural population ) and that of the occupants of Athens ( urban population ) – give value to the same package of proposed betterments. Apart from the socio-demographic composing that has motivated this sampling, another ground for that is the different manner those populations experience the debasement of the environment due to location and economic dependance on the country. One of the chief grounds why two different samples were chosen was that the purpose of the survey was to happen out usage and non-use values. As a consequence the mark population was the occupants of the Asopos River Basin, where the study took topographic point, because they would be straightforward affected by possible alterations in H2O direction. On the other manus occupants of Athinais were included in the study, because they were in close propinquity to Asopos River Basin. The study was conducted by door-to-door interviews. The interviews took topographic point in families and one grownup per house participated. Quota sampling was followed harmonizing to 2001 Census informations in order the samples to be every bit representative as possible. Finally, 25 % of the occupants were called. During the procedure of the interview were used suited showcards, which described the alternate scenario utilizing images. The census taker gave simple descriptions of the inquiries, read aloud. In that manner the census takers could break illustrate policy results to respondents in footings of properties and degrees.